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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in many tumors, which leads to chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was identified to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential purpose in promoting mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been described. Two extra splice variants had been reported but detailed nucleotide sequence information isn't yet readily available.
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Blocking peptides are peptides that bind precisely into the target antibody and block antibody binding. These peptide normally consists of the epitope acknowledged via the antibody.
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This guidebook outlines further more achievable optimization of mobile and immunoassay parameters to ensure the absolute best success are...
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This information outlines further more probable optimization of mobile and immunoassay parameters to be sure the very best results are...
Antibodies bound to the blocking peptide no longer bind for the epitope within the goal protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Concentrate on Info Cyclin E belongs into the extremely conserved cyclin household, whose members are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique get more info cyclins exhibit distinctive expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E sorts a posh with and features to be a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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